Aurintricarboxylic Acid Suppresses Hepatitis B Virus Replication by Inhibition of RNase H Activity

Background Chronic hepatitis B virus (HBV) infection is a global health threat for which there an urgent need to develop novel therapeutics. Methods Aurintricarboxylic acid (ATA) has been demonstrated with broad-spectrum antimicrobial and antiviral activities. In this study, we implemented ATA treatment in HBV-infected HBV-transfected hepatocytes uncover whether inhibits HBV replication its underlying mechanism. Results DNA levels were significantly reduced, while viral proteins or transcripts not altered. contrast, did further deteriorate DNAs, proteins, transfected by RNase H dead mutant. Moreover, showed inhibitory effect on synthesis lamivudine-resistant mutants. Conclusions potent inhibitor of disrupting the activity polymerase.